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Ciba Foundation Symposium 122 - Calcium and the Cell

This selection of displays from the Ciba origin Symposium of 1985 bargains with the principal function of calcium in intracellular approaches. Discusses regulate of intracellular calcium in addition to regulate by way of intracellular calcium, masking such subject matters as muscle contraction, metabolic strategies, hormone and transmitter secretion, membrane delivery and permeability, mobile structure and development, and the prospective contribution of calcium gradients to early embryonic improvement. comprises rigorously edited and wide (almost part the e-book) discussions of bankruptcy subject matters among energetic staff within the box on the finish of every bankruptcy.


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Much of the evidence is based on using permeabilized cells and microsomes and has been 0 Cellular + Ca2+-> M I EndODlaSmiC reticulum 11 response 1 A1 K+ FIG. 2. The role of inositol trisphosphate in calcium mobilization. Under normal conditions, agonists stimulate the hydrolysis of PtdIns4,5P2 to yield InsP,, which then releases calcium from the endoplasmic reticulum. Experimentally, InsP, can be introduced into the cell either by injection through a micropipette or by adding it to cells with permeable plasma membranes.

Nature (Lond) 242:459-461 Baylor SM, Chandler WK, Marshall MW 1983 Sarcoplasmic reticulum calcium release in frog skeletal muscle fibres estimated from arsenazo 111calcium transients. J Physiol (Lond) 344:625-666 Blinks JR, Weir W G , Hess P, Prendergast FG 1982 Measurement of Ca2+concentration in living cells. Prog Biophys Mol Biol 4O:l-114 Chandler WK, Rakowski RF, Schneider M F 1976 Effects of glycerol treatment and SCHNEIDER 32 maintained depolarization on charge movement in skeletal muscle.

Baylor et a1 (1983) used literature values for concentrations and rate constants for all known muscle calcium-binding proteins to calculate total calcium release from measured myoplasmic calcium transients. In our labora- SCHNEIDER 28 Charge Moved Free Colcium Rote of Releose 100 rns FIG. 3. Charge movement, calcium transients and rates of SR calcium release. Each line gives records for a 100 ms pulse producing the indicated membrane potential. The rate of calcium release was calculated from the calcium transient as described by Melzer et al (1984).

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