This quantity covers tracking of the biotechnological strategy with refined analytical strategies, use of the ensuing information by way of mathematical versions, and computer-aided keep an eye on for development of the productiveness of biotechnological processes.
The e-book contains 4 major elements: tools for research and biosensoring, measuring strategies, procedure types and their automation and keep watch over. using various telephone kinds, recombinant microorganisms, and reactor stipulations are provided and all current innovations of tracking and optimizing mobile progress and product formation are mentioned in regards to more desirable productivity.
issues incorporated are: equipment and tools/ Biosensors/ Characterization of Bioreactors/ decision of cellphone focus/ phone types/ Stirred Tank types/ Tower Reactor versions/ method types/ keep an eye on of Bioreactor platforms/ Automation/ regulate of Downstream Processing
Chapter 1 universal tools for procedure research and keep watch over (pages 4–25): Karl Schugerl
Chapter 2 equipment and tools in Fermentation fuel research (pages 27–74): Elmar Heinzle and Irving J. Dunn
Chapter three Biosensors (pages 75–103): Bo Mattiasson
Chapter four Characterization of Bioreactors (pages 107–148): Andreas Lubbert
Chapter five On?Line research of Broth (pages 149–178): Karl Schugerl
Chapter 6 choice of telephone focus and Characterization of Cells (pages 179–223): Kenneth F. Reardon and Thomas H. Scheper
Chapter 7 Bioreactor nation Estimation (pages 225–249): Gregory Stephanopoulos and Seujeung Park
Chapter eight Optimization of Sampling (pages 251–264): Axel Munack
Chapter nine mobilephone versions (pages 267–298): Karl?Heinz Bellgardt
Chapter 10 Stirred Tank versions (pages 299–348): Matthias Reuss and Rakesh Bajpai
Chapter eleven Tower Reactor versions (pages 349–382): Jose C. Merchuk
Chapter 12 technique types: Optimization of Yeast construction — A Case research (pages 383–406): Karl?Heinz Bellgardt and Jingqi Yuan
Chapter thirteen cardio Wastewater technique types (pages 407–439): Graham F. Andrews
Chapter 14 Anaerobic Waste Water approach types (pages 441–484): Dirk Schurbuscher and Christian Wandrey
Chapter 15 Bioprocess Kinetics and Modelling of Recombinant Fermentation (pages 485–505): Dewey D. Y. Ryu, Jeong?Yoon Kim and sunlight Bok Lee
Chapter sixteen keep an eye on of Bioreactor platforms (pages 509–560): Henry C. Lim and Kyu?Sung Lee
Chapter 17 Automation in Biotechnology (pages 561–602): Andreas Lubbert
Chapter 18 Modelling, layout, and regulate of Downstream Processing (pages 603–623): Suteaki Shioya and Ken?Ichi Suga
Chapter 19 professional platforms for Biotechnology (pages 625–636): Aarne Halme and Nazmul Karim
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Extra resources for Biotechnology: Measuring, Modelling, and Control, Volume 4, Second Edition
10 Oxygen Transfer in Large-Scale Bioreactors Large industrial fermenters can generally be expected to exhibit deviations from ideal mixing conditions. Thus, the assumptions of completely mixed gas and liquid phases may not be valid for large bioreactors, and some of the previously developed equations will not be applicable for the measurement of oxygen transfer and uptake. Little experimental information is available on concentration inhomogeneities or gradients within large bioreactors. Generally, information on the distribution of residence time is not available from which a physical and mathematical model could be established.
Feeding the recycle sys- - T a n d pH 12 Fig. 4. Reactor oxygenator recycle loop system. L R , recirculation liquid flow rate; L l =Lz,continuous liquid feed and effluent flow rate; G I , G2, gas feed and effluent flow rates; VR, reactor volume; V,, oxygenator tank volume. Mass Balancing f o r Gas Analysis tem converts it effectively into a continuous stirred tank. The well-stirred tank condition of homogeneity is fulfilled because there are only small differences in concentration between the reactor inlet and outlet.
In this figure the driving forces, calculated from Eq. (56, 57, and 5 8 ) , are shown for two different dissolved oxygen levels. Particularly noteworthy in Fig. 21. Within this range, ACAM can be used without error to calculate KLa values for large systems which exhibit oxygen gas gradients. 10 and C, = 2 mg/ Application of Gas Analysis Results to Elemental Balancing Methods L, will be only 12% lower than The use of A C A M is a striking improvement over A C W M . As can be calculated from Fig.